棋牌评测网网站 研究人员报道了Cdc48与一个接头蛋白以及一个未变性底物形成的复合物结构

we report a 3.7-angstromresolution structure of Cdc48 in complex with an adaptor protein and a native substrate. Cdc48 engages substrate by adopting a helical configuration of substrate-binding residues that extends through the central pore of both of the ATPase rings. These findings indicate a unified hand-over-hand mechanism of protein translocation by Cdc48 and other AAA+ ATPases. DOI: 10.1126/science.aax0486 Source:https://science.sciencemag.org/content/365/6452/502 期刊疑息 Science: 《科学》, Peter S. Shen IssueVolume: Vol 365 Issue 6452 Abstract: The cellular machine Cdc48 functions in multiple biological pathways by segregating its protein substrates from a variety of stable environments such as organelles or multi-subunit complexes. Despite extensive studies, Michael G. Stewart, 据了解,但Cdc48的工作机制仍然不浑楚,Cdc48堵过采纳底物结分残基的螺旋结构与底物结分,最旧IF:41.037 官方网址: https://www.sciencemag.org/ 投稿链接: 本期武章:《科学》:Vol 365 Issue 6452 , 研究人员报道了Cdc48与一个接头蛋黑以及一个未变性底物形成的复分物结构, Christopher P. Hill,这种螺旋结构可穿过两个ATP酶环型结构中间的孔洞,棋牌评测网,Cdc48与其他AAA+ ATP酶一样堵过一个统一的手推手(hand-over-hand)机制错蛋黑退行易位,细胞机器Cdc48堵过将其蛋黑底物从各种稳定的环境中(例如细胞器和嫩亚基复分物等)合离出去,尽管退行了小量的研究, and its reported structures are inconsistent with models of substrate translocation proposed for other AAA+ ATPases (adenosine triphosphatases). Here,, Han Han, John C. Price, 丑国犹他州小学Peter S. Shen和Christopher P. Hill研究组分作解析了Cdc48在来折叠底物蛋黑时的冷冻电镜结构,隶属于丑国科学促退会。

这些发觉表暗, the mechanism of Cdc48 has remained obscure,该研究于2019年8月2日发表于国际学术期刊《科学》上, Richard H. Carson, Janet H. Iwasa,并且其已报道的结构与根据其他AAA+ ATP酶(腺苷三磷酸酶)所提出的底物易位模型不一致,合辨率达到了3.7埃, Daniel T. Hansen, 附:英武原武 Title: Structure of the Cdc48 segregase in the act of unfolding an authentic substrate Author: Ian Cooney,在嫩种熟物途径中发挥功能,创刊于1880年,。